Size distribution of plant cell aggregates in batch

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May 01, 1987  The Chemical Engineering Journal, 35 (1987) B9 - B14 Size Distribution of Plant Cell Aggregates in Batch Culture F. MAVITUNA and J. M. PARK Chemical Engineering Department, UMIST, Sackuille Street, P.O. Box 88, Manchester M60 IQD (U.K.) (Received August 25, 1985; in final form October 17, 1986) B9 ABSTRACT Plant cells in suspension cultures tend to form aggregates.

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Jul 12, 2016  The aim of this work was to develop a theoretical model and its computer simulation for the prediction of plant cell aggregate size distribution change with time in batch suspension cultures. We combined the theory of the particle size distribution with the Monod kinetics to describe the plant cell aggregate growth.

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Plant cells in suspension cultures tend to form aggregates. This can affect the physiol-ogy of the cells and hence the production of valuable secondary metabolites. Aggregation is also important from a process engineering point of view. In this work, the change in the size distribution of plant cell aggregates in a batch culture is studied.

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Recent advances in the measurement of plant cell aggregate size allow for routine process monitoring of this property. We have exploited this capability to develop a conceptual model to describe changes in the aggregate size distribution that are observed over the course of a Taxus cell suspension batch

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Simulation of Size Distribution Changes of Plant Cell Aggregates during Batch Growth . By ...

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Improved assessment of aggregate size in Taxus plant cell ...

Aug 28, 2012  The valid measurement of plant cell aggregates, however, is not trivial, due to a rather large size distribution and measurement artifacts implied by the measuring method. In this study, laser diffraction was used as a novel method for characterization of Taxus chinensis cells, a major source for the antitumor agent paclitaxel.

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Modelling size distribution changes of plant cell ...

The aim of this work was to develop a theoretical model and its computer simulation for the prediction of plant cell aggregate size distribution change with time in batch suspension cultures. We combined the theory of the particle size distribution with the Monod kinetics to describe the plant cell aggregate

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Variation of aggregate size in plant cell suspension batch ...

Mar 01, 1993  Variation of aggregate size in plant cell suspension batch and semi-continuous cultures. Kieran PM, Malone DM, MacLoughlin PF. Food and Bioproducts processing., 01 Mar 1993, 71: 40-46 AGR: IND93042998 . Share this article Share with email Share with twitter Share ...

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The scale-up of plant cell culture: Engineering ...

Mavituna F Park JM (1987) Size distribution of plant cell aggregates in batch culture. Chem. Eng. J. 35: B9-B14. Google Scholar 18. Scragg AH, Bond P, Leckie F, Cresswell R, Fowler MW Allan EJ (1987) Growth and product formation by plant cell suspensions cultivated in bioreactors.

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Guidance for Industry, Q7A Good Manufacturing Practice ...

Sep 24, 2001  Batch Number (or Lot Number): A unique combination of numbers, letters, and/or symbols that identifies a batch (or lot) and from which the production and distribution

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A simple, leaky cell growth model for plant cell aggregates

A simple, leaky cell growth model for plant cell aggregates. George C. Frazier, Chemical Engineering Department, The University of Tennessee, Knoxville, Tennessee

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4 Process Involved in Scale up Criteria Microbial ...

E. Similar drop size distribution (d s = constant) ... In batch processing this bioreactor volume ranges between 500-5000 I capacities, operating over 1-3 weeks. ... The process parameters for scale up of plant cell cultures as listed in Table 8.5 demand low shear bioreactor for plant cell aggregates. As reported in literature plant cell ...

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On the Theoretical Determination of Optimal Times for ...

On the Theoretical Determination of Optimal Times for Biomass Production in Batch Cultures of Aggregate-Forming Cells . Teresa . R. Silva and F. Xavier Malcata* Escola Superior de Biotecnologia ...

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Aggregate Bins for Concrete Batching Plants Sales ...

Aggregate Bins for Concrete Batching Plants Sales Distribution. Material Handling Storage Equipment >> Aggregate Bins. FESCO Direct works primarily with JEL Concrete Plants to supply custom-built aggregate bins for small to mid-sized concrete producers throughout the US.

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Batching, Mixing, Transporting, and Handling Concrete ...

be delayed until most of the aggregate and water have intermingled in the drum. Where the mixer is charged directly from a batch plant, the materials should be added simultaneously at such rates that the charging time is about the same for all materials. If supplementary cementing materials are used, they should be added after the cement.

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Particle Size Distribution Analysis Malvern Panalytical

Molecular size, Molecular weight, Particle size, Zeta potential : Temperature range: 0°C - 120°C Particle size range: 0.3nm - 10µm Technology: Light Scattering, Dynamic Light Scattering, Electrophoretic Light Scattering, Static Light Scattering : Dispersion type: Wet : Sample cell type

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Laboratory Flotation Testing

Jun 27, 2015  Batch Laboratory Flotation Testing starts from the 3 fundamental types of flotation processes or methods which can be classed as either:. Bulk flotation; Differential flotation; Sequential flotation; While all flotation processes are selective or differential in that one mineral or group of minerals is floated away from accompanying gangue, bulk flotation generally refers to separation of ...

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Irvine to Lenexa Site Comparability Study – Dry Powder ...

The particle size distribution graph represents the variety of components contained within the cell culture formulation, with the micron size on X-axis and the quantity % on the Y-axis. In addition, a regression analysis of the particle size data was performed. The R2 value represents how close the data points are to the fitted regression line.

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Nanoparticle and macromolecular size by light scattering

Another valuable feature of batch mode DLS is its ability to extract a coarse size distribution, without any fractionation, by means of regularization analysis. While distributions calculated from batch DLS can only clearly separate populations which differ by about 3x-5x in radius, aggregates and other forms of heterogeneity may be assessed ...

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SECTION 601 CONCRETE

Ensure that the plant conforms to all safety, health, and sanitation requirements specified in Subsection 107.01.01. Supply the batching plant with bins, weighing hoppers, and scales for the fine aggregate, each size of coarse aggregate, bulk cement, and mineral admixtures. The Department will allow weighing of cementitous material cumulatively.

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Guidance for Industry, Q7A Good Manufacturing Practice ...

Sep 24, 2001  Batch Number (or Lot Number): A unique combination of numbers, letters, and/or symbols that identifies a batch (or lot) and from which the production and distribution

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Particle Size Distribution Effects that Should be ...

geometallurgy batch fl otation program often involves crushing or rolls crushing followed by ball or rod milling to achieve a target size P80. Often little attention is paid to other factors of the size distribution, namely its sharpness and whether the valuable mineral size distribution is very different to

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4 Process Involved in Scale up Criteria Microbial ...

E. Similar drop size distribution (d s = constant) ... In batch processing this bioreactor volume ranges between 500-5000 I capacities, operating over 1-3 weeks. ... The process parameters for scale up of plant cell cultures as listed in Table 8.5 demand low shear bioreactor for plant cell aggregates. As reported in literature plant cell ...

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Annex 2 W HO good manufacturing practices for active ...

6.7 Batch production record review 7. Materials management 7.1 General controls ... Storage and distribution 10.1 Warehousing procedures 10.2 Distribution procedures 11. Laboratory controls ... Note that cell substrates (mammalian, plant, insect or microbial cells, tissue or animal sources including transgenic animals) and ...

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Aggregate Bins for Concrete Batching Plants Sales ...

Aggregate Bins for Concrete Batching Plants Sales Distribution. Material Handling Storage Equipment >> Aggregate Bins. FESCO Direct works primarily with JEL Concrete Plants to supply custom-built aggregate bins for small to mid-sized concrete producers throughout the US.

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Initiation, growth and cryopreservation of plant cell ...

Aug 22, 2009  The size of cell aggregates also contributes to the accuracy of the CVS method; a relative deviation of up to 60% was observed at low FW (30 g l −1) for a Humulus lupulus cell line with ...

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Limits to biomass productivity during fed-batch ...

Nov 25, 2020  This study compared the growth of Laminaria saccharina female gametophyte filamentous cell suspension cultures in a stirred-tank photobioreactor under batch and fed-batch nutrient addition modes over a 48-day cultivation period. Cultures were grown on GP2 artificial seawater medium (0.75 mM nitrate, N:P = 16:1) at pH 8.3 without iron or copper. Total equivalent nutrient loadings

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Vaccine Manufacturing - PubMed Central (PMC)

Jul 17, 2017  Viruses are grown in cells, which can be either primary cells, such as chicken fibroblasts (e.g., yellow fever vaccine), or continuous cell lines, such as MRC-5 (e.g., hepatitis A vaccine). Bacterial pathogens are grown in bioreactors using medium developed to optimize the yield of the antigen while maintaining its integrity.

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Population analysis of a commercial Saccharomyces ...

Feb 01, 2011  Counts and the cell size distribution were determined by electric particle analysis using a Multisizer II electric particle analyser (Coulter Corporation, Miami, FL), with an aperture tube 30 μm in diameter and a processing capacity of 500 μL of cell suspension in 0.9% NaCl, filtered previously through a 0.2-μm membrane filter.

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Vulcan Materials - Facilities Map

Contact. Vulcan Materials Company Corporate Office 1200 Urban Center Drive Birmingham, AL 35242 Tel: (205) 298-3000 Questions about our site? Contact Us

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Particle Size Distribution Analysis Malvern Panalytical

Molecular size, Molecular weight, Particle size, Zeta potential : Temperature range: 0°C - 120°C Particle size range: 0.3nm - 10µm Technology: Light Scattering, Dynamic Light Scattering, Electrophoretic Light Scattering, Static Light Scattering : Dispersion type: Wet : Sample cell type

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Laboratory Flotation Testing

Jun 27, 2015  Batch Laboratory Flotation Testing starts from the 3 fundamental types of flotation processes or methods which can be classed as either:. Bulk flotation; Differential flotation; Sequential flotation; While all flotation processes are selective or differential in that one mineral or group of minerals is floated away from accompanying gangue, bulk flotation generally refers to separation of ...

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Preparation of Batch Manufacturing Record (BMR ...

1. Batch Record: A very first page of the BMR has all records about the batch as batch number, batch size, composition, master formula record referred the weight of the batch, shelf life, storage conditions, manufacturing license number, manufacturing date, expiry date, date of starting and date of completion.

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Questions and Answers on Current Good Manufacturing ...

Mycoplasma contain only a cell membrane and have no cell wall. They are not susceptible to beta-lactams and do not take up Gram stain. ... varying in size from 0.2 to 0.3 microns or smaller ...

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